Metagenomic Analysis of the Rhizosphere Soil Microbiome with Respect to Phytic Acid Utilization
نویسندگان
چکیده
While phytic acid is a major form of organic phosphate in many soils, plant utilization of phytic acid is normally limited; however, culture trials of Lotus japonicus using experimental field soil that had been managed without phosphate fertilizer for over 90 years showed significant usage of phytic acid applied to soil for growth and flowering and differences in the degree of growth, even in the same culture pot. To understand the key metabolic processes involved in soil phytic acid utilization, we analyzed rhizosphere soil microbial communities using molecular ecological approaches. Although molecular fingerprint analysis revealed changes in the rhizosphere soil microbial communities from bulk soil microbial community, no clear relationship between the microbiome composition and flowering status that might be related to phytic acid utilization of L. japonicus could be determined. However, metagenomic analysis revealed changes in the relative abundance of the classes Bacteroidetes, Betaproteobacteria, Chlorobi, Dehalococcoidetes and Methanobacteria, which include strains that potentially promote plant growth and phytic acid utilization, and some gene clusters relating to phytic acid utilization, such as alkaline phosphatase and citrate synthase, with the phytic acid utilization status of the plant. This study highlights phylogenetic and metabolic features of the microbial community of the L. japonicus rhizosphere and provides a basic understanding of how rhizosphere microbial communities affect the phytic acid status in soil.
منابع مشابه
Utilization of Phytic Acid by Cooperative Interaction in Rhizosphere
Plants and microbes interact in various relationships which have both adverse and beneficial effects on plant growth and microbial communities. Many processes of these interactions occur at or near the root–soil interface, which is known as the rhizosphere. The rhizosphere is defined as the soil region under the immediate influence of plant roots. Many studies suggest that the microbial communi...
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